秦川牛CD36基因多态性检测及其与肉用性状的相关性研究

doi:10.11843/j.issn.0366-6964.2014.07.005

Abstract

Abstract:

The aim of the study was to research polymorphism of fatty acid translocase gene (Cluster of differentiation 36，CD36) and analyze its effect on meat traits，then find molecular breeding methods of improving meat traits in Qinchuan cattle.In this study，288 24-month-old Qinchuan heifers under similar feeding conditions were randomly selected.Direct DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were used to genotype the polymorphisms of CD36 gene.The polymorphisms of bovine CD36 gene and its associations with meat traits (back fat thickness，eye muscle area，eye muscle depth and intramuscular fatty content) were analyzed.Four mutation sites were detected，named SV1，SV2，SV3，and SV4，respectively.Further，the linkage disequilibrium and haplotypes were analyzed among the four loci，suggested that SV1 and SV2 were complete linkage，SV1-SV2 and SV3 were strong linked，and SV4 was little linked with SV1-SV2 and SV3，respectively.Haplotype of ITA was dominant (with frequency of 60.4%).Except differentiation of eye muscle depth at SV4，the result of association analysis showed significant or extremely significant difference between different genotypes of single and combined sites in intramuscular fatty content in Qinchuan cattle，and the individuals with combined genotype IITTAG had a lower intramuscular fatty content than that of the individuals with combined genotype IITTAG (P<0.05) and combined genotype WWCCAA (P<0.01).As the result showed，CD36 gene had a significant effect on meat traits in Qinchuan cattle，and reducing the ratio of individuals with combined genotype IITTAG may be a method to improve meat traits.

CLC Number:

S813

ZHANG Song，ZAN Lin-sen，WANG Hong-bao，WANG Guo-qing，FU Chang-zhen，ZHANG Ya-ran，MEI Chu-gang，DENG Guan-qun. Association of CD36 Gene Polymorphism with Meat Traits in Qinchuan Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(7): 1060-1067.

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References

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Association of CD36 Gene Polymorphism with Meat Traits in Qinchuan Cattle

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